assay data analyzer software Search Results


tc20 data analyzer  (Bio-Rad)
99
Bio-Rad tc20 data analyzer
PKP4 modulates keratinocyte proliferation (A) Levels of PKP4 protein in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h or 72h in medium with or without Ca 2+ . Left: Representative western blots of PKP4. GAPDH was used as a loading control. Right: Quantification of PKP4 protein amounts normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. (B) Measurement of proliferation using an IncuCyte S3 system. Left: Representative live cell images of WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for up to 96h in LCM. Scale bar = 100 μm. Right: Area occupied by cell nuclei at the indicated time points, as determined from live cell images. Data are shown relative to measurements at the beginning of recording (time zero). Graphs represent average values from three independent experiments. (C) The diameter of trypsinized WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in LCM automatically measured using the BioRad <t>TC20</t> Cell Counter. Top: Representative live cell images. Scale bar = 100 μm. Bottom: Quantification of cell size. Averages +SD from three independent experiments are plotted. n ≥ 1,000 cells per condition. (D) Propidium iodide-labeling and flow cytometry analysis of the cell cycle. Number of WT and PKP4-KO cells grown for 24h in LCM or HCM in G0/G1 phase, S phase, or G2/M phase. Averages +SD from three independent experiments are plotted. (E) BrdU incorporation in WT and PKP4-KO cells grown for 24h, 48h, or 72h in LCM or HCM. Averages +SD from three independent experiments are plotted. (F) Levels of proliferation markers in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in medium with or without Ca 2+ . Left: Schematic of cell cycle dependent expression of RB and H3. Since RB is phosphorylated in late G1 phase, thereby releasing E2F1 and promoting its transcriptional activity, phospho-RB was used as a marker for G1-S phase transition. Phosphorylation of H3 served as an M phase marker. Mid: Representative western blots of phospho-RB and phospho-H3. GAPDH was used as a loading control. Right: Quantification of phosphorylation levels normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. Quantification of LCM versus HCM treatment has been omitted. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, not significant. Significance was determined by one-way ANOVA with Tukey’s multiple comparisons test (A, B, C, and F) or by student’s unpaired two-tailed t-test (D and E).
Tc20 Data Analyzer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/assay+data+analyzer+software/TC20+Data+Analyzer+Software/pmc11402648-115-0-4
Average 99 stars, based on 1 article reviews
tc20 data analyzer - by Bioz Stars, 2026-10
99/100 stars
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advanced image data analyzer software  (Raytest GmbH)
90
Raytest GmbH advanced image data analyzer software
PKP4 modulates keratinocyte proliferation (A) Levels of PKP4 protein in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h or 72h in medium with or without Ca 2+ . Left: Representative western blots of PKP4. GAPDH was used as a loading control. Right: Quantification of PKP4 protein amounts normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. (B) Measurement of proliferation using an IncuCyte S3 system. Left: Representative live cell images of WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for up to 96h in LCM. Scale bar = 100 μm. Right: Area occupied by cell nuclei at the indicated time points, as determined from live cell images. Data are shown relative to measurements at the beginning of recording (time zero). Graphs represent average values from three independent experiments. (C) The diameter of trypsinized WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in LCM automatically measured using the BioRad <t>TC20</t> Cell Counter. Top: Representative live cell images. Scale bar = 100 μm. Bottom: Quantification of cell size. Averages +SD from three independent experiments are plotted. n ≥ 1,000 cells per condition. (D) Propidium iodide-labeling and flow cytometry analysis of the cell cycle. Number of WT and PKP4-KO cells grown for 24h in LCM or HCM in G0/G1 phase, S phase, or G2/M phase. Averages +SD from three independent experiments are plotted. (E) BrdU incorporation in WT and PKP4-KO cells grown for 24h, 48h, or 72h in LCM or HCM. Averages +SD from three independent experiments are plotted. (F) Levels of proliferation markers in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in medium with or without Ca 2+ . Left: Schematic of cell cycle dependent expression of RB and H3. Since RB is phosphorylated in late G1 phase, thereby releasing E2F1 and promoting its transcriptional activity, phospho-RB was used as a marker for G1-S phase transition. Phosphorylation of H3 served as an M phase marker. Mid: Representative western blots of phospho-RB and phospho-H3. GAPDH was used as a loading control. Right: Quantification of phosphorylation levels normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. Quantification of LCM versus HCM treatment has been omitted. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, not significant. Significance was determined by one-way ANOVA with Tukey’s multiple comparisons test (A, B, C, and F) or by student’s unpaired two-tailed t-test (D and E).
Advanced Image Data Analyzer Software, supplied by Raytest GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/assay+data+analyzer+software/advanced+image+data+analyzer+software/10__1074_slash_jbc__m111__231977-112-9-14
Average 90 stars, based on 1 article reviews
advanced image data analyzer software - by Bioz Stars, 2026-10
90/100 stars
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aida image analyzer software  (FUJIFILM)
90
FUJIFILM aida image analyzer software
PKP4 modulates keratinocyte proliferation (A) Levels of PKP4 protein in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h or 72h in medium with or without Ca 2+ . Left: Representative western blots of PKP4. GAPDH was used as a loading control. Right: Quantification of PKP4 protein amounts normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. (B) Measurement of proliferation using an IncuCyte S3 system. Left: Representative live cell images of WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for up to 96h in LCM. Scale bar = 100 μm. Right: Area occupied by cell nuclei at the indicated time points, as determined from live cell images. Data are shown relative to measurements at the beginning of recording (time zero). Graphs represent average values from three independent experiments. (C) The diameter of trypsinized WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in LCM automatically measured using the BioRad <t>TC20</t> Cell Counter. Top: Representative live cell images. Scale bar = 100 μm. Bottom: Quantification of cell size. Averages +SD from three independent experiments are plotted. n ≥ 1,000 cells per condition. (D) Propidium iodide-labeling and flow cytometry analysis of the cell cycle. Number of WT and PKP4-KO cells grown for 24h in LCM or HCM in G0/G1 phase, S phase, or G2/M phase. Averages +SD from three independent experiments are plotted. (E) BrdU incorporation in WT and PKP4-KO cells grown for 24h, 48h, or 72h in LCM or HCM. Averages +SD from three independent experiments are plotted. (F) Levels of proliferation markers in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in medium with or without Ca 2+ . Left: Schematic of cell cycle dependent expression of RB and H3. Since RB is phosphorylated in late G1 phase, thereby releasing E2F1 and promoting its transcriptional activity, phospho-RB was used as a marker for G1-S phase transition. Phosphorylation of H3 served as an M phase marker. Mid: Representative western blots of phospho-RB and phospho-H3. GAPDH was used as a loading control. Right: Quantification of phosphorylation levels normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. Quantification of LCM versus HCM treatment has been omitted. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, not significant. Significance was determined by one-way ANOVA with Tukey’s multiple comparisons test (A, B, C, and F) or by student’s unpaired two-tailed t-test (D and E).
Aida Image Analyzer Software, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/assay+data+analyzer+software/aida+advanced+image+data+analyzer+software/10__1074_slash_jbc__m304720200-64-59-55
Average 90 stars, based on 1 article reviews
aida image analyzer software - by Bioz Stars, 2026-10
90/100 stars
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aida advanced image data analyzer software, v4.19.029  (Raytest GmbH)
90
Raytest GmbH aida advanced image data analyzer software, v4.19.029
PKP4 modulates keratinocyte proliferation (A) Levels of PKP4 protein in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h or 72h in medium with or without Ca 2+ . Left: Representative western blots of PKP4. GAPDH was used as a loading control. Right: Quantification of PKP4 protein amounts normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. (B) Measurement of proliferation using an IncuCyte S3 system. Left: Representative live cell images of WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for up to 96h in LCM. Scale bar = 100 μm. Right: Area occupied by cell nuclei at the indicated time points, as determined from live cell images. Data are shown relative to measurements at the beginning of recording (time zero). Graphs represent average values from three independent experiments. (C) The diameter of trypsinized WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in LCM automatically measured using the BioRad <t>TC20</t> Cell Counter. Top: Representative live cell images. Scale bar = 100 μm. Bottom: Quantification of cell size. Averages +SD from three independent experiments are plotted. n ≥ 1,000 cells per condition. (D) Propidium iodide-labeling and flow cytometry analysis of the cell cycle. Number of WT and PKP4-KO cells grown for 24h in LCM or HCM in G0/G1 phase, S phase, or G2/M phase. Averages +SD from three independent experiments are plotted. (E) BrdU incorporation in WT and PKP4-KO cells grown for 24h, 48h, or 72h in LCM or HCM. Averages +SD from three independent experiments are plotted. (F) Levels of proliferation markers in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in medium with or without Ca 2+ . Left: Schematic of cell cycle dependent expression of RB and H3. Since RB is phosphorylated in late G1 phase, thereby releasing E2F1 and promoting its transcriptional activity, phospho-RB was used as a marker for G1-S phase transition. Phosphorylation of H3 served as an M phase marker. Mid: Representative western blots of phospho-RB and phospho-H3. GAPDH was used as a loading control. Right: Quantification of phosphorylation levels normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. Quantification of LCM versus HCM treatment has been omitted. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, not significant. Significance was determined by one-way ANOVA with Tukey’s multiple comparisons test (A, B, C, and F) or by student’s unpaired two-tailed t-test (D and E).
Aida Advanced Image Data Analyzer Software, V4.19.029, supplied by Raytest GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/assay+data+analyzer+software/aida+software+advanced+image+data+analyzer+2+11/pmc04536213-64-19-20
Average 90 stars, based on 1 article reviews
aida advanced image data analyzer software, v4.19.029 - by Bioz Stars, 2026-10
90/100 stars
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nano analyze software package version 3.11.0  (TA Instruments)
90
TA Instruments nano analyze software package version 3.11.0
PKP4 modulates keratinocyte proliferation (A) Levels of PKP4 protein in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h or 72h in medium with or without Ca 2+ . Left: Representative western blots of PKP4. GAPDH was used as a loading control. Right: Quantification of PKP4 protein amounts normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. (B) Measurement of proliferation using an IncuCyte S3 system. Left: Representative live cell images of WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for up to 96h in LCM. Scale bar = 100 μm. Right: Area occupied by cell nuclei at the indicated time points, as determined from live cell images. Data are shown relative to measurements at the beginning of recording (time zero). Graphs represent average values from three independent experiments. (C) The diameter of trypsinized WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in LCM automatically measured using the BioRad <t>TC20</t> Cell Counter. Top: Representative live cell images. Scale bar = 100 μm. Bottom: Quantification of cell size. Averages +SD from three independent experiments are plotted. n ≥ 1,000 cells per condition. (D) Propidium iodide-labeling and flow cytometry analysis of the cell cycle. Number of WT and PKP4-KO cells grown for 24h in LCM or HCM in G0/G1 phase, S phase, or G2/M phase. Averages +SD from three independent experiments are plotted. (E) BrdU incorporation in WT and PKP4-KO cells grown for 24h, 48h, or 72h in LCM or HCM. Averages +SD from three independent experiments are plotted. (F) Levels of proliferation markers in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in medium with or without Ca 2+ . Left: Schematic of cell cycle dependent expression of RB and H3. Since RB is phosphorylated in late G1 phase, thereby releasing E2F1 and promoting its transcriptional activity, phospho-RB was used as a marker for G1-S phase transition. Phosphorylation of H3 served as an M phase marker. Mid: Representative western blots of phospho-RB and phospho-H3. GAPDH was used as a loading control. Right: Quantification of phosphorylation levels normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. Quantification of LCM versus HCM treatment has been omitted. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, not significant. Significance was determined by one-way ANOVA with Tukey’s multiple comparisons test (A, B, C, and F) or by student’s unpaired two-tailed t-test (D and E).
Nano Analyze Software Package Version 3.11.0, supplied by TA Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/assay+data+analyzer+software/software+nano+analyze+data+analysis+version+3+11+0/pm38248705-60-23-21
Average 90 stars, based on 1 article reviews
nano analyze software package version 3.11.0 - by Bioz Stars, 2026-10
90/100 stars
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advanced image data analyzer 2d v3.11 software  (Raytest GmbH)
90
Raytest GmbH advanced image data analyzer 2d v3.11 software
PKP4 modulates keratinocyte proliferation (A) Levels of PKP4 protein in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h or 72h in medium with or without Ca 2+ . Left: Representative western blots of PKP4. GAPDH was used as a loading control. Right: Quantification of PKP4 protein amounts normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. (B) Measurement of proliferation using an IncuCyte S3 system. Left: Representative live cell images of WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for up to 96h in LCM. Scale bar = 100 μm. Right: Area occupied by cell nuclei at the indicated time points, as determined from live cell images. Data are shown relative to measurements at the beginning of recording (time zero). Graphs represent average values from three independent experiments. (C) The diameter of trypsinized WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in LCM automatically measured using the BioRad <t>TC20</t> Cell Counter. Top: Representative live cell images. Scale bar = 100 μm. Bottom: Quantification of cell size. Averages +SD from three independent experiments are plotted. n ≥ 1,000 cells per condition. (D) Propidium iodide-labeling and flow cytometry analysis of the cell cycle. Number of WT and PKP4-KO cells grown for 24h in LCM or HCM in G0/G1 phase, S phase, or G2/M phase. Averages +SD from three independent experiments are plotted. (E) BrdU incorporation in WT and PKP4-KO cells grown for 24h, 48h, or 72h in LCM or HCM. Averages +SD from three independent experiments are plotted. (F) Levels of proliferation markers in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in medium with or without Ca 2+ . Left: Schematic of cell cycle dependent expression of RB and H3. Since RB is phosphorylated in late G1 phase, thereby releasing E2F1 and promoting its transcriptional activity, phospho-RB was used as a marker for G1-S phase transition. Phosphorylation of H3 served as an M phase marker. Mid: Representative western blots of phospho-RB and phospho-H3. GAPDH was used as a loading control. Right: Quantification of phosphorylation levels normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. Quantification of LCM versus HCM treatment has been omitted. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, not significant. Significance was determined by one-way ANOVA with Tukey’s multiple comparisons test (A, B, C, and F) or by student’s unpaired two-tailed t-test (D and E).
Advanced Image Data Analyzer 2d V3.11 Software, supplied by Raytest GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/assay+data+analyzer+software/advanced+image+data+analyzer+software+version+3+51/pmc00522783-292-12-19
Average 90 stars, based on 1 article reviews
advanced image data analyzer 2d v3.11 software - by Bioz Stars, 2026-10
90/100 stars
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advanced image data analyzer software v4.5  (Raytek Scientific Limited)
90
Raytek Scientific Limited advanced image data analyzer software v4.5
PKP4 modulates keratinocyte proliferation (A) Levels of PKP4 protein in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h or 72h in medium with or without Ca 2+ . Left: Representative western blots of PKP4. GAPDH was used as a loading control. Right: Quantification of PKP4 protein amounts normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. (B) Measurement of proliferation using an IncuCyte S3 system. Left: Representative live cell images of WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for up to 96h in LCM. Scale bar = 100 μm. Right: Area occupied by cell nuclei at the indicated time points, as determined from live cell images. Data are shown relative to measurements at the beginning of recording (time zero). Graphs represent average values from three independent experiments. (C) The diameter of trypsinized WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in LCM automatically measured using the BioRad <t>TC20</t> Cell Counter. Top: Representative live cell images. Scale bar = 100 μm. Bottom: Quantification of cell size. Averages +SD from three independent experiments are plotted. n ≥ 1,000 cells per condition. (D) Propidium iodide-labeling and flow cytometry analysis of the cell cycle. Number of WT and PKP4-KO cells grown for 24h in LCM or HCM in G0/G1 phase, S phase, or G2/M phase. Averages +SD from three independent experiments are plotted. (E) BrdU incorporation in WT and PKP4-KO cells grown for 24h, 48h, or 72h in LCM or HCM. Averages +SD from three independent experiments are plotted. (F) Levels of proliferation markers in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in medium with or without Ca 2+ . Left: Schematic of cell cycle dependent expression of RB and H3. Since RB is phosphorylated in late G1 phase, thereby releasing E2F1 and promoting its transcriptional activity, phospho-RB was used as a marker for G1-S phase transition. Phosphorylation of H3 served as an M phase marker. Mid: Representative western blots of phospho-RB and phospho-H3. GAPDH was used as a loading control. Right: Quantification of phosphorylation levels normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. Quantification of LCM versus HCM treatment has been omitted. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, not significant. Significance was determined by one-way ANOVA with Tukey’s multiple comparisons test (A, B, C, and F) or by student’s unpaired two-tailed t-test (D and E).
Advanced Image Data Analyzer Software V4.5, supplied by Raytek Scientific Limited, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/assay+data+analyzer+software/advanced+image+data+analyzer+software+v4+5/pm22858986-46-4-10
Average 90 stars, based on 1 article reviews
advanced image data analyzer software v4.5 - by Bioz Stars, 2026-10
90/100 stars
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advanced image data analyzer software  (FUJIFILM)
90
FUJIFILM advanced image data analyzer software
PKP4 modulates keratinocyte proliferation (A) Levels of PKP4 protein in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h or 72h in medium with or without Ca 2+ . Left: Representative western blots of PKP4. GAPDH was used as a loading control. Right: Quantification of PKP4 protein amounts normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. (B) Measurement of proliferation using an IncuCyte S3 system. Left: Representative live cell images of WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for up to 96h in LCM. Scale bar = 100 μm. Right: Area occupied by cell nuclei at the indicated time points, as determined from live cell images. Data are shown relative to measurements at the beginning of recording (time zero). Graphs represent average values from three independent experiments. (C) The diameter of trypsinized WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in LCM automatically measured using the BioRad <t>TC20</t> Cell Counter. Top: Representative live cell images. Scale bar = 100 μm. Bottom: Quantification of cell size. Averages +SD from three independent experiments are plotted. n ≥ 1,000 cells per condition. (D) Propidium iodide-labeling and flow cytometry analysis of the cell cycle. Number of WT and PKP4-KO cells grown for 24h in LCM or HCM in G0/G1 phase, S phase, or G2/M phase. Averages +SD from three independent experiments are plotted. (E) BrdU incorporation in WT and PKP4-KO cells grown for 24h, 48h, or 72h in LCM or HCM. Averages +SD from three independent experiments are plotted. (F) Levels of proliferation markers in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in medium with or without Ca 2+ . Left: Schematic of cell cycle dependent expression of RB and H3. Since RB is phosphorylated in late G1 phase, thereby releasing E2F1 and promoting its transcriptional activity, phospho-RB was used as a marker for G1-S phase transition. Phosphorylation of H3 served as an M phase marker. Mid: Representative western blots of phospho-RB and phospho-H3. GAPDH was used as a loading control. Right: Quantification of phosphorylation levels normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. Quantification of LCM versus HCM treatment has been omitted. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, not significant. Significance was determined by one-way ANOVA with Tukey’s multiple comparisons test (A, B, C, and F) or by student’s unpaired two-tailed t-test (D and E).
Advanced Image Data Analyzer Software, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/assay+data+analyzer+software/advanced+image+data+analyzer+software/10__1128_slash_mcb__24__5__2190___2201__2004-87-9-14
Average 90 stars, based on 1 article reviews
advanced image data analyzer software - by Bioz Stars, 2026-10
90/100 stars
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advanced data image analyzer software aida vers4.26.038  (Raytest GmbH)
90
Raytest GmbH advanced data image analyzer software aida vers4.26.038
PKP4 modulates keratinocyte proliferation (A) Levels of PKP4 protein in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h or 72h in medium with or without Ca 2+ . Left: Representative western blots of PKP4. GAPDH was used as a loading control. Right: Quantification of PKP4 protein amounts normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. (B) Measurement of proliferation using an IncuCyte S3 system. Left: Representative live cell images of WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for up to 96h in LCM. Scale bar = 100 μm. Right: Area occupied by cell nuclei at the indicated time points, as determined from live cell images. Data are shown relative to measurements at the beginning of recording (time zero). Graphs represent average values from three independent experiments. (C) The diameter of trypsinized WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in LCM automatically measured using the BioRad <t>TC20</t> Cell Counter. Top: Representative live cell images. Scale bar = 100 μm. Bottom: Quantification of cell size. Averages +SD from three independent experiments are plotted. n ≥ 1,000 cells per condition. (D) Propidium iodide-labeling and flow cytometry analysis of the cell cycle. Number of WT and PKP4-KO cells grown for 24h in LCM or HCM in G0/G1 phase, S phase, or G2/M phase. Averages +SD from three independent experiments are plotted. (E) BrdU incorporation in WT and PKP4-KO cells grown for 24h, 48h, or 72h in LCM or HCM. Averages +SD from three independent experiments are plotted. (F) Levels of proliferation markers in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in medium with or without Ca 2+ . Left: Schematic of cell cycle dependent expression of RB and H3. Since RB is phosphorylated in late G1 phase, thereby releasing E2F1 and promoting its transcriptional activity, phospho-RB was used as a marker for G1-S phase transition. Phosphorylation of H3 served as an M phase marker. Mid: Representative western blots of phospho-RB and phospho-H3. GAPDH was used as a loading control. Right: Quantification of phosphorylation levels normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. Quantification of LCM versus HCM treatment has been omitted. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, not significant. Significance was determined by one-way ANOVA with Tukey’s multiple comparisons test (A, B, C, and F) or by student’s unpaired two-tailed t-test (D and E).
Advanced Data Image Analyzer Software Aida Vers4.26.038, supplied by Raytest GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/assay+data+analyzer+software/advanced+data+image+analyzer+software+aida+vers4+26+038/pmc04923876-153-13-20
Average 90 stars, based on 1 article reviews
advanced data image analyzer software aida vers4.26.038 - by Bioz Stars, 2026-10
90/100 stars
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advanced image data analyzer software v. 3.4  (Raytest GmbH)
90
Raytest GmbH advanced image data analyzer software v. 3.4
PKP4 modulates keratinocyte proliferation (A) Levels of PKP4 protein in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h or 72h in medium with or without Ca 2+ . Left: Representative western blots of PKP4. GAPDH was used as a loading control. Right: Quantification of PKP4 protein amounts normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. (B) Measurement of proliferation using an IncuCyte S3 system. Left: Representative live cell images of WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for up to 96h in LCM. Scale bar = 100 μm. Right: Area occupied by cell nuclei at the indicated time points, as determined from live cell images. Data are shown relative to measurements at the beginning of recording (time zero). Graphs represent average values from three independent experiments. (C) The diameter of trypsinized WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in LCM automatically measured using the BioRad <t>TC20</t> Cell Counter. Top: Representative live cell images. Scale bar = 100 μm. Bottom: Quantification of cell size. Averages +SD from three independent experiments are plotted. n ≥ 1,000 cells per condition. (D) Propidium iodide-labeling and flow cytometry analysis of the cell cycle. Number of WT and PKP4-KO cells grown for 24h in LCM or HCM in G0/G1 phase, S phase, or G2/M phase. Averages +SD from three independent experiments are plotted. (E) BrdU incorporation in WT and PKP4-KO cells grown for 24h, 48h, or 72h in LCM or HCM. Averages +SD from three independent experiments are plotted. (F) Levels of proliferation markers in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in medium with or without Ca 2+ . Left: Schematic of cell cycle dependent expression of RB and H3. Since RB is phosphorylated in late G1 phase, thereby releasing E2F1 and promoting its transcriptional activity, phospho-RB was used as a marker for G1-S phase transition. Phosphorylation of H3 served as an M phase marker. Mid: Representative western blots of phospho-RB and phospho-H3. GAPDH was used as a loading control. Right: Quantification of phosphorylation levels normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. Quantification of LCM versus HCM treatment has been omitted. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, not significant. Significance was determined by one-way ANOVA with Tukey’s multiple comparisons test (A, B, C, and F) or by student’s unpaired two-tailed t-test (D and E).
Advanced Image Data Analyzer Software V. 3.4, supplied by Raytest GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/assay+data+analyzer+software/advanced+image+data+analyzer+software+v++3+4/us08703136-443-8-15
Average 90 stars, based on 1 article reviews
advanced image data analyzer software v. 3.4 - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
movisens dataanalyzer software  (Movisens GmbH)
90
Movisens GmbH movisens dataanalyzer software
PKP4 modulates keratinocyte proliferation (A) Levels of PKP4 protein in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h or 72h in medium with or without Ca 2+ . Left: Representative western blots of PKP4. GAPDH was used as a loading control. Right: Quantification of PKP4 protein amounts normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. (B) Measurement of proliferation using an IncuCyte S3 system. Left: Representative live cell images of WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for up to 96h in LCM. Scale bar = 100 μm. Right: Area occupied by cell nuclei at the indicated time points, as determined from live cell images. Data are shown relative to measurements at the beginning of recording (time zero). Graphs represent average values from three independent experiments. (C) The diameter of trypsinized WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in LCM automatically measured using the BioRad <t>TC20</t> Cell Counter. Top: Representative live cell images. Scale bar = 100 μm. Bottom: Quantification of cell size. Averages +SD from three independent experiments are plotted. n ≥ 1,000 cells per condition. (D) Propidium iodide-labeling and flow cytometry analysis of the cell cycle. Number of WT and PKP4-KO cells grown for 24h in LCM or HCM in G0/G1 phase, S phase, or G2/M phase. Averages +SD from three independent experiments are plotted. (E) BrdU incorporation in WT and PKP4-KO cells grown for 24h, 48h, or 72h in LCM or HCM. Averages +SD from three independent experiments are plotted. (F) Levels of proliferation markers in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in medium with or without Ca 2+ . Left: Schematic of cell cycle dependent expression of RB and H3. Since RB is phosphorylated in late G1 phase, thereby releasing E2F1 and promoting its transcriptional activity, phospho-RB was used as a marker for G1-S phase transition. Phosphorylation of H3 served as an M phase marker. Mid: Representative western blots of phospho-RB and phospho-H3. GAPDH was used as a loading control. Right: Quantification of phosphorylation levels normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. Quantification of LCM versus HCM treatment has been omitted. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, not significant. Significance was determined by one-way ANOVA with Tukey’s multiple comparisons test (A, B, C, and F) or by student’s unpaired two-tailed t-test (D and E).
Movisens Dataanalyzer Software, supplied by Movisens GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/assay+data+analyzer+software/movisens+data+analyzer+software/10__1007_slash_s11818___016___0087___z-2990-8-7
Average 90 stars, based on 1 article reviews
movisens dataanalyzer software - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
proteus 61 software package  (NETZSCH)
90
NETZSCH proteus 61 software package
PKP4 modulates keratinocyte proliferation (A) Levels of PKP4 protein in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h or 72h in medium with or without Ca 2+ . Left: Representative western blots of PKP4. GAPDH was used as a loading control. Right: Quantification of PKP4 protein amounts normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. (B) Measurement of proliferation using an IncuCyte S3 system. Left: Representative live cell images of WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for up to 96h in LCM. Scale bar = 100 μm. Right: Area occupied by cell nuclei at the indicated time points, as determined from live cell images. Data are shown relative to measurements at the beginning of recording (time zero). Graphs represent average values from three independent experiments. (C) The diameter of trypsinized WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in LCM automatically measured using the BioRad <t>TC20</t> Cell Counter. Top: Representative live cell images. Scale bar = 100 μm. Bottom: Quantification of cell size. Averages +SD from three independent experiments are plotted. n ≥ 1,000 cells per condition. (D) Propidium iodide-labeling and flow cytometry analysis of the cell cycle. Number of WT and PKP4-KO cells grown for 24h in LCM or HCM in G0/G1 phase, S phase, or G2/M phase. Averages +SD from three independent experiments are plotted. (E) BrdU incorporation in WT and PKP4-KO cells grown for 24h, 48h, or 72h in LCM or HCM. Averages +SD from three independent experiments are plotted. (F) Levels of proliferation markers in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in medium with or without Ca 2+ . Left: Schematic of cell cycle dependent expression of RB and H3. Since RB is phosphorylated in late G1 phase, thereby releasing E2F1 and promoting its transcriptional activity, phospho-RB was used as a marker for G1-S phase transition. Phosphorylation of H3 served as an M phase marker. Mid: Representative western blots of phospho-RB and phospho-H3. GAPDH was used as a loading control. Right: Quantification of phosphorylation levels normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. Quantification of LCM versus HCM treatment has been omitted. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, not significant. Significance was determined by one-way ANOVA with Tukey’s multiple comparisons test (A, B, C, and F) or by student’s unpaired two-tailed t-test (D and E).
Proteus 61 Software Package, supplied by NETZSCH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/assay+data+analyzer+software/proteus+61+data+analyzing+software/10__1002_slash_zaac__202000484-258-7-6
Average 90 stars, based on 1 article reviews
proteus 61 software package - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

Image Search Results


PKP4 modulates keratinocyte proliferation (A) Levels of PKP4 protein in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h or 72h in medium with or without Ca 2+ . Left: Representative western blots of PKP4. GAPDH was used as a loading control. Right: Quantification of PKP4 protein amounts normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. (B) Measurement of proliferation using an IncuCyte S3 system. Left: Representative live cell images of WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for up to 96h in LCM. Scale bar = 100 μm. Right: Area occupied by cell nuclei at the indicated time points, as determined from live cell images. Data are shown relative to measurements at the beginning of recording (time zero). Graphs represent average values from three independent experiments. (C) The diameter of trypsinized WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in LCM automatically measured using the BioRad TC20 Cell Counter. Top: Representative live cell images. Scale bar = 100 μm. Bottom: Quantification of cell size. Averages +SD from three independent experiments are plotted. n ≥ 1,000 cells per condition. (D) Propidium iodide-labeling and flow cytometry analysis of the cell cycle. Number of WT and PKP4-KO cells grown for 24h in LCM or HCM in G0/G1 phase, S phase, or G2/M phase. Averages +SD from three independent experiments are plotted. (E) BrdU incorporation in WT and PKP4-KO cells grown for 24h, 48h, or 72h in LCM or HCM. Averages +SD from three independent experiments are plotted. (F) Levels of proliferation markers in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in medium with or without Ca 2+ . Left: Schematic of cell cycle dependent expression of RB and H3. Since RB is phosphorylated in late G1 phase, thereby releasing E2F1 and promoting its transcriptional activity, phospho-RB was used as a marker for G1-S phase transition. Phosphorylation of H3 served as an M phase marker. Mid: Representative western blots of phospho-RB and phospho-H3. GAPDH was used as a loading control. Right: Quantification of phosphorylation levels normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. Quantification of LCM versus HCM treatment has been omitted. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, not significant. Significance was determined by one-way ANOVA with Tukey’s multiple comparisons test (A, B, C, and F) or by student’s unpaired two-tailed t-test (D and E).

Journal: iScience

Article Title: A feedback loop between plakophilin 4 and YAP signaling regulates keratinocyte differentiation

doi: 10.1016/j.isci.2024.110762

Figure Lengend Snippet: PKP4 modulates keratinocyte proliferation (A) Levels of PKP4 protein in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h or 72h in medium with or without Ca 2+ . Left: Representative western blots of PKP4. GAPDH was used as a loading control. Right: Quantification of PKP4 protein amounts normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. (B) Measurement of proliferation using an IncuCyte S3 system. Left: Representative live cell images of WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for up to 96h in LCM. Scale bar = 100 μm. Right: Area occupied by cell nuclei at the indicated time points, as determined from live cell images. Data are shown relative to measurements at the beginning of recording (time zero). Graphs represent average values from three independent experiments. (C) The diameter of trypsinized WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in LCM automatically measured using the BioRad TC20 Cell Counter. Top: Representative live cell images. Scale bar = 100 μm. Bottom: Quantification of cell size. Averages +SD from three independent experiments are plotted. n ≥ 1,000 cells per condition. (D) Propidium iodide-labeling and flow cytometry analysis of the cell cycle. Number of WT and PKP4-KO cells grown for 24h in LCM or HCM in G0/G1 phase, S phase, or G2/M phase. Averages +SD from three independent experiments are plotted. (E) BrdU incorporation in WT and PKP4-KO cells grown for 24h, 48h, or 72h in LCM or HCM. Averages +SD from three independent experiments are plotted. (F) Levels of proliferation markers in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in medium with or without Ca 2+ . Left: Schematic of cell cycle dependent expression of RB and H3. Since RB is phosphorylated in late G1 phase, thereby releasing E2F1 and promoting its transcriptional activity, phospho-RB was used as a marker for G1-S phase transition. Phosphorylation of H3 served as an M phase marker. Mid: Representative western blots of phospho-RB and phospho-H3. GAPDH was used as a loading control. Right: Quantification of phosphorylation levels normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. Quantification of LCM versus HCM treatment has been omitted. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, not significant. Significance was determined by one-way ANOVA with Tukey’s multiple comparisons test (A, B, C, and F) or by student’s unpaired two-tailed t-test (D and E).

Article Snippet: TC20 data analyzer , BioRad , https://www.bio-rad.com/webroot/web/html/lsr/products/cell_counting/product_overlay/global/tc20-data-ana.html.

Techniques: Western Blot, Control, Labeling, Flow Cytometry, BrdU Incorporation Assay, Expressing, Activity Assay, Marker, Sublimation, Phospho-proteomics, Two Tailed Test

Journal: iScience

Article Title: A feedback loop between plakophilin 4 and YAP signaling regulates keratinocyte differentiation

doi: 10.1016/j.isci.2024.110762

Figure Lengend Snippet:

Article Snippet: TC20 data analyzer , BioRad , https://www.bio-rad.com/webroot/web/html/lsr/products/cell_counting/product_overlay/global/tc20-data-ana.html.

Techniques: Transduction, Virus, Recombinant, Formulation, Random Hexamer, Reverse Transcription, Western Blot, Enzyme-linked Immunosorbent Assay, Bicinchoninic Acid Protein Assay, Expressing, Mutagenesis, Plasmid Preparation, Software